Nanopore Technology

Nanopore stochastic sensing has received wide attention as a label-free and versatile technique for measuring single molecules. By monitoring the ionic current modulations produced by the passage of target analytes through a single nanopore bathed in high salt solutions at a fixed applied potential, both the concentration and the identity of the analyte can be determined. One significant advantage of nanopore stochastic sensing over most of other detection techniques is its multidimensional signal, in which multiple parameters can be obtained from a single measurement. Therefore, simultaneous detection and quantification of multiple components in a solution mixture can be readily accomplished.  LBNano has developed three types of nanopore sensors, which are based on biological protein ion channels, and synthetic nanoscale-sized pores fabricated with polymeric films and silicon nitride chips, respectively. During these years, LBNano has invented various innovative strategies for detection of a wide variety of chemical and biochemical species and to explore various applications.

Fluorescence resonance energy transfer (FRET), which relies on the distance-dependent transfer of energy from a donor molecule to an acceptor molecule, has been widely used to study molecular interaction and biomolecule conformational change as well as to develop biosensors due to its high sensitivity, simplicity and reproducibility. By taking advantage of graphene, a novel two-dimensional one-atom-thick carbon material, LBNano pioneered a multiplexing fluorescence sensor platform for simultaneous measurement of multiple biomarkers, especially for cancer monitoring.  Furthermore, by using artificial intelligence and machine learning, our technology not only provides highly accurate cancer diagnosis and prognosis, but also enable differentiation of cancer types.